Microorganisms are living beings. which can only be visualized under a microscope, they can have effeCts on human health and can be transmitted from one person to another through hands, fluids or poor hygiene practice. We can find them on solid surfaces (work table, door knobs, etc.), on the clothes (lab coat) hands and gloves, in the air. etc. Identification is important because they can be contaminating and contagious, causing pathologies in humans. Hand washing is an important element in the control of the transmission, contamination and infections, caused by these agents. Gram-negative bacteria were identified and their sensitivity patterns were determined. Automated equipment and tests of high sensitivity and specificity were used for the identification of pathogenic or opportunistic species and their sensitivity was determined according to the Kirby Bauer method. It was possible to identify species of Escgerichia, Klebsiella, Enterobacter, Proteus and Pseudomona, which showed sensitivity to Ceftriaxone, Ampicillin, Ciprofloxacin and being resistant to Amoxicillin with Glabulanic acid in a low percentage. Areas contaminated with microorganisms that could affect the health of those using these areas of research were identified. Inadequate laboratory development or practice in these contaminated sites can become a potential contaminant that can lead to an invasive process and affect the biosecurity of the personnel working there. Biosecurity standards and handling of infectious-contagious samples should be implemented to avoid occupational hazards. Key words: Microbiology, environment, microorganism, hygiene, biosecurity