The problem of solid waste is an issue of global importance due to the socio-environmental and unsanitary conditions that influence negatively the quality of life of citizens and the ecosystem. The current reality forces to implement a kind of strategies that contemplate the investigation of microorganisms as well as their capacity to biodegrade these residues. Bacteriological contamination was characterized in the solid waste generated within the central field of the Autonomous University of Chiriquí, Province of Chiriquí, Panama. This study was classified with a mixed approach, correlational descriptive scope and non-experimental design. The sample corresponded to 306 bags of solid waste collected in five randomly places. The methods of analysis were divided into field and laboratory sampling. The techniques used were quartering and identification of enterobacteria present in these residues. It was obtained as results that the bacterium with more frequency was Klebsiella sp. with 33%, Escherichia coli 29%, Enterobacter sp. 19%, Proteus vulgaris 10%, in lower percentage Pseudomonas and Acinetobacter with 5% each. In conclusion, the research hypothesis that the bacteriological load is not influenced by the physical parameters humidity, density and temperature in university solid waste was validated.